期刊
NEURODEGENERATIVE DISEASES
卷 17, 期 4-5, 页码 213-226出版社
KARGER
DOI: 10.1159/000475467
关键词
Huntington disease; Large-animal model; Mutant huntingtin; Spermatozoa; Mitochondrial metabolism
资金
- Norwegian Financial Mechanism
- Ministry of Education, Youth and Sports [MSMT-28477/2014, 7F14308]
- MSMT [NPU 1609]
- CHDI Foundation [A-8248, A-5378]
- PRVOUK [P24/205024-4]
- GACR [14-36804G]
- Centre of Mitochondrial Biology and Pathology (MITOCENTRE)
- [UNCE 204011]
- [SVV UK 260256/2016]
Background: Huntington disease (HD) is a fatal neurode-generative disorder involving reduced muscle coordination, mental and behavioral changes, and testicular degeneration. In order to further clarify the decreased fertility and penetration ability of the spermatozoa of transgenic HD minipig boars (TgHD), we applied a set of mitochondrial metabolism (MM) parameter measurements to this promising biological material, which can be collected noninvasively in longitudinal studies. Objective: We aimed to optimize methods for MM measurements in spermatozoa and to establish possible biomarkers of HD in TgHD spermatozoa expressing the N-terminal part of mutated human huntingtin. Methods: Semen samples from 12 TgHD and wild-type animals, aged 12-65 months, were obtained repeatedly during the study. Respiration was measured by polarography, MM was assessed by the detection of oxidation of radiolabeled substrates (mitochondrial energy-generating system; MEGS), and the content of the oxidative phosphorylation system subunits was detected by Western blot. Three possibly interfering factors were statistically analyzed: the effect of HD, generation and aging. Results: We found 5 MM parameters which were significantly diminished in TgHD spermatozoa and propose 3 specific MEGS incubations and complex I-dependent respiration as potential biomarkers of HD in TgHD spermatozoa. Conclusions: Our results suggest a link between the gain of toxic function of mutated huntingtin in TgHD spermatozoa and the observed MM and/or glycolytic impairment. We determined 4 biomarkers useful for HD phenotyping and experimental therapy monitoring studies in TgHD minipigs. (C) 2017 S. Karger AG, Basel
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