期刊
NEUROBIOLOGY OF AGING
卷 60, 期 -, 页码 116-128出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.neurobiolaging.2017.08.028
关键词
Beta amyloid; ApoA-I; ApoA-I-Milano; Alzheimer's disease; APP23; Neuroinflammation
资金
- Instituto de Salud Carlos III (ISCIII) [PI14/01134]
- European Regional Development Fund, (FEDER)
- Fundacio La Marato de TV3 [40/U/2014]
- EU Joint Programmee-Neurodegenerative Disease Research (JPND) project, through ISCIII, Spain (SNOWBALL)
- ISCIII, Spain [CP12/03259]
Beyond the crucial role of apolipoprotein A-I (ApoA-I) on peripheral cholesterol metabolism, this apolipoprotein has also been implicated in beta amyloid (A beta) erelated neuropathologies. ApoA-I-Milano (M) is a mutated variant, which showed increased vasoprotective properties compared to ApoA-I-wild type in models of atherosclerosis and cardiovascular damage. We speculated that ApoA-I-M may also protect A beta-affected vasculature and reverse some of the pathological features associated with Alzheimer's disease (AD). For this purpose, we produced and characterized human recombinant ApoA-I-wild type and ApoA-I-M proteins. Both of them were able to avoid the aggregation of A beta in vitro, even though recombinant ApoA-I-M was significantly more effective in protecting endothelial cells from A beta(1-42)-toxicity. Next, we determined the effect of chronic intravenous administration of rApoA-I-M in the APP23-transgenic mouse model of AD. We found reduced cerebral A beta levels in mice that received rApoA-I-M, which were accompanied by a lower expression of astrocyte and microglia neuro-inflammatory markers. Our results suggest an applicability of this molecule as a therapeutic candidate for protecting the brain in AD. (C) 2017 Elsevier Inc. All rights reserved.
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