期刊
BIO-PROTOCOL
卷 8, 期 6, 页码 -出版社
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2774
关键词
Reactive oxygen species; 2 ',7 '-Dichlorofluorescein diacetate; C. elegans; Antioxidant
类别
资金
- National Research Foundation of South Korea [NRF-2017R1C1B5015695]
- Brody Brothers Endowment Grant [21602-664261]
- NIH [1R15GM112174-01A1]
- NSF [MCB1714264]
- National Institutes of Health - Office of Research Infrastructure Programs [P40 OD010440]
Reactive oxygen species (ROS) are generated during normal metabolic processes under aerobic conditions. Since ROS production initiates harmful radical chain reactions on cellular macromolecules, including lipid peroxidation, DNA mutation, and protein denaturation, it has been implicated in a wide spectrum of diseases such as cancer, cardiovascular disease, ischemia-reperfusion and aging. Over the past several decades, antioxidants have received explosive attention regarding their protective potential against these deleterious reactions. Accordingly, many analytical methodologies have been developed for the evaluation of the antioxidant capacity of compounds or complex biological samples. Herein, we introduce a simple and convenient method to detect in vivo intracellular ROS levels photometrically in Caenorhabditis elegans using 2',7'-dichlorofluorescein diacetate (H(2)DCFDA), a cell permeant tracer.
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