期刊
BIO-PROTOCOL
卷 8, 期 8, 页码 -出版社
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2823
关键词
Primary cultures; Striatum dissection; Striatal neurons; Medium spiny neurons
类别
资金
- European community FEDER fund through the 'Programa Operacional Factores de Competitividade' - COMPETE 2020
- 'Fundacao para a Ciencia e a Tecnologia' (FCT), Portugal [POCI-01-0145-FEDER-007440]
- Teva Pharmaceutical Industries Ltd.
Primary cultures of murine striatal neurons are widely used to explore cellular mechanisms in neurobiology, including brain diseases. Here we describe a detailed and standardized protocol to dissect and culture embryonic murine striatal neurons GABA-positive/DARPP-32-positive for 12 days in vitro, when they show good neuronal cell connectivity and the presence of dendritic spines, which reflects the maturation of the network.
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