期刊
ELECTROPHORESIS
卷 36, 期 9-10, 页码 1228-1233出版社
WILEY
DOI: 10.1002/elps.201500043
关键词
Human serum; Immunoproteomics; Saliva; S; epidermidis biofilm
资金
- FCT [SFRH/BD/78235/2011]
- Fundacao para a Ciencia e a Tecnologia (FCT)
- COMPETE grants [PTDC/BIA-MIC/113450/2009, FCOMP-01-0124-FEDER-014309]
- QOPNA research unit [PEst-C/QUI/UI0062/2013]
- RNEM (National Mass Spetrometry Network)
- FCT Strategic Project [PEst-OE/EQB/LA0023/2013]
- Programa Operacional Regional do Norte (ON.2 - O Novo Norte), QREN, FEDER [NORTE-07-0124-FEDER-000027]
- project Consolidating Research Expertise and Resources on Cellular and Molecular Biotechnology at CEB/IBB [FCOMP-01-0124-FEDER-027462]
- [CENTRO-07-ST24-FEDER-002034]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/78235/2011] Funding Source: FCT
Saliva is essential to interact with microorganisms in the oral cavity. Therefore, the interest in saliva antimicrobial properties is on the rise. Here, we used an immunoproteomic approach, based on protein separation of Staphylococcus epidermidis biofilms by 2DE, followed by Western-blotting, to compare human serum and saliva reactivity profile. A total of 17 proteins were identified by MALDI-TOF/TOF. Serum and saliva presented a distinct pattern of immunoreactive proteins. Our results suggest that saliva seems to have higher propensity to react against S. epidermidis proteins with oxidoreductase activity and proteins involved with L-serine metabolic processes. We show that saliva was a powerful tool for the identification of potential S. epidermidis biofilms proteins.
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