期刊
BIO-PROTOCOL
卷 8, 期 21, 页码 -出版社
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.3073
关键词
Lentivirus; Stable cell lines; Gene knockdown; Gene over-expression; Mammalian cell lines; Transduction
类别
资金
- NIH [CA-67166, CA-198291, CA-1 97713]
- Sydney Frank Foundation
- Kimmelman Fund
Lentiviruses are used very widely to generate stable expression mammalian cell lines. They are used for both gene down-regulation (by using shRNA) or for gene up-regulation (by using ORF of gene of interest). The technique of generating stable cell lines using 3rd generation lentivirus is very robust and it typically takes about 1-2 weeks to get stable expression for most mammalian cell lines. The advantage of using the 3rd generation lentivirus are that are very safe and they are replication incompetent.
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