期刊
NATURE PHOTONICS
卷 11, 期 5, 页码 322-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NPHOTON.2017.55
关键词
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资金
- European Research Council [336716]
- Research Council of Norway [244764/F11]
- German Academic Exchange Service [57160327]
- German Federal Ministry for Economic Affairs and Energy [KF2140610NT4]
Present optical nanoscopy techniques use a complex microscope for imaging and a simple glass slide to hold the sample. Here, we demonstrate the inverse: the use of a complex, but mass-producible optical chip, which hosts the sample and provides a waveguide for the illumination source, and a standard low-cost microscope to acquire super-resolved images via two different approaches. Waveguides composed of a material with high refractive-index contrast provide a strong evanescent field that is used for single-molecule switching and fluorescence excitation, thus enabling chip-based single-molecule localization microscopy. Additionally, multimode interference patterns induce spatial fluorescence intensity variations that enable fluctuation-based super-resolution imaging. As chip-based nanoscopy separates the illumination and detection light paths, total-internal-reflection fluorescence excitation is possible over a large field of view, with up to 0.5 mm x 0.5 mm being demonstrated. Using multicolour chip-based nanoscopy, we visualize fenestrations in liver sinusoidal endothelial cells.
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