期刊
ACS APPLIED NANO MATERIALS
卷 1, 期 7, 页码 3590-3599出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsanm.8b00737
关键词
gold nanoparticles; self-assembly; outer membrane proteins; single chain variable fragment; LSPR; lateral flow assay; biosensing
资金
- BBSRC Industrial CASE Award
- Orla Protein Technologies Ltd.
- Wellcome Trust [064345]
Functional protein-gold nanoparticle (AuNP) conjugates have a wide variety of applications including biosensing and drug delivery. Correct protein orientation, which is important to maintain functionality on the nanoparticle surface, can be difficult to achieve in practice, and dedicated protein scaffolds have been used on planar gold surfaces to drive the self-assembly of oriented protein arrays. Here we use the transmembrane domain of Escherichia coli outer membrane protein A (OmpA(TM)) to create protein-AuNP conjugates. The addition of a single cysteine residue into a periplasmic loop, to create cysOmpA(TM), drives oriented assembly and increased equilibrium binding. As the protein surface concentration increases, the sulfur gold bond in cysOmpA(TM) creates a more densely populated AuNP surface than the poorly organized wtOmpA(TM) layer. The functionalization of AuNP improved both their stability and homogeneity. This was further exploited using multidomain protein chimeras, based on cysOmpA(TM), which were shown to form ordered protein arrays with their functional domains displayed away from the AuNP surface. A fusion with protein G was shown to specifically bind antibodies via their Fc region. Next, an in vitro selected single chain antibody (scFv)-cysOmpA(TM) fusion protein, bound to AuNP, detected influenza A nucleoprotein, a widely used antigen in diagnostic assays. Finally, using the same scFv-cysOmpA(TM)-AuNP conjugates, a prototype lateral flow assay for influenza demonstrated the utility of fully recombinant self-assembling sensor layers. By simultaneously removing the need for both animal antibodies and a separate immobilization procedure, this technology could greatly simplify the development of a range of in vitro diagnostics.
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