期刊
NATURE NANOTECHNOLOGY
卷 12, 期 5, 页码 453-459出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NNANO.2017.23
关键词
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资金
- NSFC [NSFC 21521063, NSFC 21327009]
- US National Institutes of Health [GM079359, CA133086]
- Key Project of the Major Research Plan of NSFC [91130004]
- NSFC A3 Project [11421110002]
- NSFC Tianyuan Projects [11426235, 11526211]
- NSFC Innovative Group Fund [11621101]
- US NSF FRG [DMS-0968360]
Cells interact with the extracellular environment through molecules expressed on the membrane. Disruption of these membrane-bound interactions (or encounters) can result in disease progression. Advances in super-resolution microscopy have allowed membrane encounters to be examined, however, these methods cannot image entire membranes and cannot provide information on the dynamic interactions between membrane-bound molecules. Here, we show a novel DNA probe that can transduce transient membrane encounter events into readable cumulative fluorescence signals. The probe, which translocates from one anchor site to another, mimicking motor proteins, is realized through a toehold-mediated DNA strand displacement reaction. Using this probe, we successfully monitored rapid encounter events of membrane lipid domains using flow cytometry and fluorescence microscopy. Our results show a preference for encounters within the same lipid domains.
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