期刊
NATURE NANOTECHNOLOGY
卷 12, 期 10, 页码 974-979出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NNANO.2017.134
关键词
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资金
- NIGMS/NINR [R01GM07718507, R01GM10801402, R01NR01567601, R01NR01389804, R01NS42617]
- NIBIB [R21EB017539]
- NSF [NSEC EEC-0914790, 1325114]
- National Center for the Advancing Translational Sciences [UL1TR001070]
- NINDS [R21NS099869]
- NIDDK Diabetic Complications Consortium (DiaComp) [DK076169 (U24DK076169)]
- NIH [R21EB017539, R01HL132355, R01NS099869, R01DK076566]
- Ohio State University Center for Regenerative Medicine and Cell Based Therapies
- Nanoscale Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices
- Directorate For Engineering
- Div Of Civil, Mechanical, & Manufact Inn [1325114] Funding Source: National Science Foundation
Although cellular therapies represent a promising strategy for a number of conditions, current approaches face major translational hurdles, including limited cell sources and the need for cumbersome pre-processing steps (for example, isolation, induced pluripotency)(1-6). In vivo cell reprogramming has the potential to enable more-effective cell-based therapies by using readily available cell sources (for example, fibroblasts) and circumventing the need for ex vivo pre-processing(7,8). Existing reprogramming methodologies, however, are fraught with caveats, including a heavy reliance on viral transfection(9,10). Moreover, capsid size constraints and/ or the stochastic nature of status quo approaches (viral and non- viral) pose additional limitations, thus highlighting the need for safer and more deterministic in vivo reprogramming methods(11,12). Here, we report a novel yet simple-to-implement non-viral approach to topically reprogram tissues through a nanochannelled device validated with well-established and newly developed reprogramming models of induced neurons and endothelium, respectively. We demonstrate the simplicity and utility of this approach by rescuing necrotizing tissues and whole limbs using two murine models of injury-induced ischaemia.
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