期刊
NATURE METHODS
卷 14, 期 8, 页码 793-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.4347
关键词
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资金
- NIGMS [P41-GM103311]
- Agency for Science, Technology and Research Singapore
- Leona M. and Harry B. Helmsley Charitable Trust [2017-PG-MED001]
- US National Institutes of Health (NIH) [DP5 OD021396-01]
- Jane Coffin Child's Foundation postdoctoral fellowship
- National Institute of Aging K99 transitional award [AG050749]
- National Institute of General Medical Sciences [GM103310, GM053757]
- Simons Foundation [349247]
We present a strategy for tackling preferred specimen orientation in single-particle cryogenic electron microscopy by employing tilts during data collection. We also describe a tool to quantify the resulting directional resolution using 3D Fourier shell correlation volumes. We applied these methods to determine the structures at near-atomic resolution of the influenza hemagglutinin trimer, which adopts a highly preferred specimen orientation, and of ribosomal biogenesis intermediates, which adopt moderately preferred orientations.
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