期刊
NATURE METHODS
卷 14, 期 6, 页码 577-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.4286
关键词
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资金
- NIH Office of the Director (OD)
- National Institute of Dental & Craniofacial Research (NIDCR)
- Department of Defense Breast Cancer Research Breakthrough Program
- Pew Charitable Trusts
- Alfred P. Sloan Foundation
- Quantitative Bioscience Institute (QBI)
- J. David Gladstone Biofulcrum Program
- NIH [RO1 DA036858, NIH U01EB021240, DOD W81XWH-17-1-0018, P50 GM082250, U19 AI106754, P01 HL089707, RO1 GM084279, U19 AI118610, RO1 AI120694]
- Pew Scholar Fellowship
- Alfred P. Sloan Fellowship
- [DP5 0D017887]
We describe a combinatorial CRISPR interference (CRISPRi) screening platform for mapping genetic interactions in mammalian cells. We targeted 107 chromatin -regulation factors in human cells with pools of either single or double single guide RNAs (sgRNAs) to downregulate individual genes or gene pairs, respectively. Relative enrichment analysis of individual sgRNAs or sgRNA pairs allowed for quantitative characterization of genetic interactions, and comparison with protein -protein -interaction data revealed a functional map of chromatin regulation.
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