期刊
NATURE METHODS
卷 14, 期 3, 页码 267-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.4145
关键词
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资金
- McNair Scholarship
- McNair Single Cell Initiative
The quantification of transcriptional variation in single cells, particularly within the same cell population, is currently limited by the low sensitivity and high technical noise of single-cell RNA-seq assays. We report multiple annealing and dC-tailing-based quantitative single-cell RNA-seq (MATQ-seq), a highly sensitive and quantitative method for single-cell sequencing of total RNA. By systematically determining technical noise, we show that MATQ-seq captures genuine biological variation between whole transcriptomes of single cells.
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