期刊
NATURE CHEMICAL BIOLOGY
卷 13, 期 8, 页码 839-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nchembio.2410
关键词
-
资金
- NIH [R37 AI091476, P01 AI100263]
Cpf1 is a CRISPR effector protein that has greater specificity than Streptococcus pyogenes Cas9 (SpCas9) in genome-editing applications. Here we show that Lachnospiraceae bacterium (Lb) and Acidaminococus sp. (As) Cpf1 orthologs have RNase activities that can excise multiple CRISPR RNAs (crRNAs) from a single RNA polymerase II-driven RNA transcript expressed in mammalian cells. This property simplifies modification of multiple genomic targets and can be used to increase the efficiency of Cpf1-mediated editing.
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