期刊
NATURE CELL BIOLOGY
卷 19, 期 10, 页码 1153-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb3607
关键词
-
类别
资金
- American Society of Hematology
- Hartwell Foundation
- NIDDK [K01DK080846, R01DK104028]
- American Lebanese Syrian Associated Charities (ALSAC)
- NCI [P30 CA021765-35]
- National Cancer Institute at the National Institute of Health [P30 CA21765]
- ALSAC
- NCI (SJCRH Cell & Tissue Imaging Center)
Current dogma asserts that mammalian lifelong blood production is established by a small number of blood progenitors. However, this model is based on assays that require the disruption, transplantation and/or culture of embryonic tissues. Here, we used the sample-to-sample variance of a multicoloured lineage trace reporter to assess the frequency of emerging lifelong blood progenitors while avoiding the disruption, culture or transplantation of embryos. We find that approximately 719 Flk1(+) mesodermal precursors, 633 VE-cadherin(+) endothelial precursors and 545 Vav1(+) nascent blood stem and progenitor cells emerge to establish the haematopoietic system at embryonic days (E) 7-E8.5, E8.5-E11.5 and E11.5-E14.5, respectively. We also determined that the spatio-temporal recruitment of endothelial blood precursors begins at E8.5 and ends by E10.5, and that many c-Kit(+) clusters of newly specified blood progenitors in the aorta are polyclonal in origin. Our work illuminates the dynamics of the developing mammalian blood system during homeostasis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据