期刊
NATURAL PRODUCT RESEARCH
卷 32, 期 15, 页码 1867-1871出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2017.1405405
关键词
Linum usitatissimum; hairy root culture; Agrobacterium rhizogenes; lignans; phenolic acids; antioxidant capacity
Hairy root culture is a promising alternative method for the production of secondary metabolites. In this study, transformed root of Linum usitatissimum was established using Agrobacterium rhizogenes A4 strain from root cultures for lignans, phenolic acids and antioxidant capacity determination. Total lignin content (secoisolariciresinol diglucoside, secoisolariciresinol and matairesinol) was 55.5% higher in transformed root cultures than in the non-transformed root culture. Secoisolariciresinol was detected in higher concentration (2.107 mu mol/g DM) in the transformed root culture than non-transformed culture (1.099 mu mol/g DM). Secoisolariciresinol diglucoside and matairesinol were exclusively detected in the transformed root culture, but were not found in the non-transformed root culture. The overall production of phenolic acids in transformed roots was approximately 3.5 times higher than that of the corresponding non-transformed culture. Free radical scavenging DPPH and ABTS(+) assays showed 2.9-fold and 1.76-fold higher anti-oxidant activity in transformed root culture as compared to non-transformed.
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