Chemical constituents of Eleutherococcus sessiliflorus extract and its sedative-hypnotic effect

The present study was designed to investigate the chemical constituents and bioactivities of the roots of Eleutherococcus sessiliflorus (E. sessiliflorus). The compounds were isolated through various chromatography techniques and elucidated by mass spectrometric (MS), 1D- and 2D-NMR analyses. The sedative-hypnotic effect of E. sessiliflorus ethanol extract and its fractions (acetic ether, n-butanol and water fraction) were also investigated. In the chemical constituent study, one new compound, 3-ethyl-5-hydroxy-3-(hydroxymethyl) pentyl-3-(3,4-dihydroxyphenyl) acrylate and fourteen known compounds were isolated and identified. The chromatographic fingerprint of E. sessiliflorus was established by UPLC-PDA-MS/MS analysis. In bioactivity study, it was found that the water fraction of E. sessiliflorus extract could prolong pentobarbital-induced sleeping time more than that of the other fractions of E. sessiliflorus extract in mice, which provided a basis for future study on sedative-hypnotic effect of the chemical constituents in E. sessiliflorus.