期刊
NATURAL PRODUCT RESEARCH
卷 32, 期 23, 页码 2766-2774出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2017.1378211
关键词
Pistacia genus; essential oil; ITS; 5S-rRNA-NTS; antifungal activity
资金
- Co.S.Me.Se
This work reports the results and the comparison concerning the chemical and biomolecular analyses and the antifungal activity of three wild Pistacia species (Anacardiaceae) from Sardinia. Volatile oils from leaves and twigs of Pistacia x saportae, Pistacia lentiscus and Pistacia terebinthus were characterised using GC-FID and GCMS techniques and tested against some fungal strains. Two DNA nuclear regions (ITS and 5S-rRNA-NTS) were amplified through PCR technique and sequenced. The three Pistacia have similar chemical profile, although there are some important quantitative differences. The analysis of ITS and 5S-rRNA-NTS regions reveals a species-specific nucleotide variation among the three taxa. This method could emerge as a powerful tool for the species identification, especially because the discrimination of these three taxa appears difficult for non-expert botanists. Concerning the antifungal activity, P. lentiscus and P. x saportae show the highest activity against Cryptococcus neoformans, with a MIC value of 0.32 mu L/mL. [GRAPHICS] .
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