期刊
NANOSCALE
卷 9, 期 44, 页码 17561-17570出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7nr06019j
关键词
-
类别
资金
- Natural Science Foundation of China [21476165, 21606166, 21621004]
- 863 Program of China [2013AA102204]
- Natural Science Foundation of Tianjin [16JCYBJC19600]
- Ministry of Science and Technology of China [2012YQ090194]
- Beiyang Young Scholar of the Tianjin University
Metal-organic frameworks (MOFs) have drawn extensive research interest as candidates for enzyme immobilization owing to their tunable porosity, high surface area, and excellent chemical/thermal stability. Herein, we report a facile and universal strategy for enzyme immobilization using highly stable hierarchically porous metal-organic frameworks (HP-MOFs). The HP-MOFs were stable over a wide pH range (pH = 2-11 for HP-DUT-5) and met the catalysis conditions of most enzymes. The as-prepared hierarchical micro/mesoporous MOFs with mesoporous defects showed a superior adsorption capacity towards enzymes. The maximum adsorption capacity of HP-DUT-5 for glucose oxidase (GOx) and uricase was 208 mg g(-1) and 225 mg g(-1), respectively. Furthermore, we constructed two multi-enzyme biosensors for glucose and uric acid (UA) by immobilizing GOx and uricase with horseradish peroxidase (HRP) on HP-DUT-5, respectively. These sensors were efficiently applied in the colorimetric detection of glucose and UA and showed good sensitivity, selectivity, and recyclability.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据