期刊
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
卷 13, 期 3, 页码 835-841出版社
ELSEVIER
DOI: 10.1016/j.nano.2016.11.016
关键词
Nanomedicine; Minimal- and non-invasive diagnostics; Extracellular vesicles; Exosomes; Raman spectroscopy; Infrared spectroscopy
资金
- Innovation Fond Medicine of the Universitaetsklinikum Freiburg
- ERA-RusPlus ID110 Exodiagnos/BMWi ZIM [KF2979902CR4]
- BMBF [IB-GUS/RUS 01DJ15026]
In cancer, extracellular vesicles (EV) contribute to tumor progression by regulating local and systemic effects. Being released into body fluids, EV may be used in nanomedicine as a valuable source for diagnostic biomarkers. In this work, infrared and Raman spectroscopy were used for comprehensive comparative analysis of cancer versus non-cancer EV and patient screening. Two different EV fractions enriched in exosomes and microvesicles were isolated by differential centrifugation from serum and plasma of cancer and non-cancer patients and from serum and plasma of a healthy donor. The EV fractions were then subjected to drop-coating deposition and drying on calcium fluoride substrates. Reduction of alpha-helix-rich proteins and enhancement of beta-sheet-rich proteins as a cancer-specific blood EV signature was determined, and subsequently this feature was applied for a pilot study aiming to detect prostate cancer in a test cohort of patients with high-grade prostate carcinoma and benign hypoplasia. (C) 2016 Elsevier Inc. All rights reserved.
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