期刊
NANOMEDICINE
卷 12, 期 20, 页码 2503-2517出版社
FUTURE MEDICINE LTD
DOI: 10.2217/nnm-2017-0181
关键词
angiopep-2; blood-brain barrier; drug delivery; gold nanoparticle; gold nanorods; photothermal effect; theranostics
资金
- CONICYT, Ministry of Education of Chile: Fondap [15130011]
- Fondecyt [1170929, 1130425, BIO2016-75327-R, 21110304, 21120617, 21151400, 11130494]
- MINECO [CTQ2015-67870-P]
- Generalitat de Catalunya (Spain) [2014 SGR 137]
Aim: To improve the in vivo delivery of gold nanorods (GNRs) to the central nervous system of rats, these gold nanoparticles were conjugated to Angiopep-2, a shuttle peptide that can cross the blood-brain barrier. Materials & methods: GNRs were synthesized and modified using polyethylene glycol and Angiopep-2 (GNR-PEG-Angiopep-2). The physicochemical properties, in vitro cytotoxicity and ex vivo biodistribution of the conjugate were examined. Results: GNR-PEG-Angiopep-2 was stable over the following days, and the different concentrations that were tested did not affect the viability of microvascular endothelial cells. The conjugation of Angiopep-2 to GNRs enhanced the endocytosis of these particles (in vitro) and the accumulation in brains (in vivo), when compared with GNRs modified only with PEG. Conclusion: This study provides evidence that Angiopep-2 improves the delivery of GNRs to the brain parenchyma. This property is highly relevant for future applications of GNRs as platforms for photothermal and theranostic purposes.
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