期刊
NANO LETTERS
卷 17, 期 2, 页码 1277-1281出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.6b05040
关键词
Interferometric scattering; balanced interference; label-free; single particle tracking; single-molecule detection; superresolution; iSCAT; digital holography; interference microscopy
类别
资金
- European Commission (ERC Adv. Grant) [670949-LightNet]
- MINECO Severo Ochoa Programme for Centres of Excellence in RD [SEV-2015-0522, FIS2012-35527, FIS2015-69258-P]
- Catalan AGAUR [2014 SGR01540]
- CERCA Programme of Generalitat de Catalunya
- Fundacio CELLEX (Barcelona)
- Marie-Curie International Fellowship COFUND
- ICREA Funding Source: Custom
Label-free detection, analysis, and rapid tracking of nanop articles is crucial for future ultrasensitive sensing applications, ranging from understanding of biological interactions to the study of size-dependent classical-quantum transitions. Yet optical techniques to distinguish nanoparticles directly among their background remain challenging. Here we present amplified interferometric scattering microscopy (a-iSCAT) as a new all-optical method capable of detecting individual nanoparticles as small as 15 kDa proteins that is equivalent to half a GFP. By balancing scattering and reflection amplitudes the interference contrast of the nanopartide signal is amplified 1 to 2 orders of magnitude. Beyond high sensitivity, a-iSCAT allows high-speed image acquisition exceeding several hundreds of frames-per-second. We showcase the performance of our approach by detecting single Streptavidin binding events and by tracking single Ferritin proteins at 400 frames-per-second with 12 nm localization precision over seconds. Moreover, due to its extremely simple experimental realization, this advancement finally enables a cheap and routine implementation of label-free all-optical single nanopartide detection platforms with sensitivity operating at the single protein level.
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