期刊
NANO LETTERS
卷 17, 期 11, 页码 7117-7124出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.7b03918
关键词
Cell separation; intracellular marker; nanoneedle array; single-cell array; iPS cells; high-throughput
类别
资金
- Japan Society for the Promotion of Science (JSPS) through the Funding Program for Next Generation World-Leading Researchers (NEXT Program)
- Council for Science and Technology Policy (CSTP)
- Grants-in-Aid for Scientific Research [16K04888] Funding Source: KAKEN
Focusing on intracellular targets, we propose a new cell separation technique based on a nano needle array (NNA) device, which allows simultaneous insertion of multiple needles into multiple cells. The device is designed to target and lift (fish) individual cells from a mixed population of cells on a substrate using an antibody-functionalized NNA. The mechanics underlying this approach were validated by force analysis using an atomic force microscope. Accurate high-throughput separation was achieved using one-to-one contacts between the nano needles and the cells by preparing a single-cell array in which the positions of the cells were aligned with 10,000 nano needles in the NNA. Cell-type-specific separation was realized by controlling the adhesion force so that the cells could be detached in cell-type-independent manner. Separation of nestin-expressing neural stem cells (NSCs) derived from human induced pluripotent stem cells (hiPSCs) was demonstrated using the proposed technology, and successful differentiation to neuronal cells was confirmed.
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