期刊
MYCOLOGICAL PROGRESS
卷 16, 期 4, 页码 311-323出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11557-016-1262-0
关键词
Bioactive metabolites; Cultivation; Edible mushroom; Molecular data; Morphology
类别
资金
- German Academic Exchange Service (DAAD)
- Thai Royal Golden Ph.D. Jubilee-Industry program (RGJ) for a joint TRF-DAAD PPP academic exchange grant
- RGJ for a personal stipend [Ph.D/0138/2553, 4.S.MF/53/A.3]
- University of Wyoming
- NSF [DEB-1050292]
- Division Of Environmental Biology
- Direct For Biological Sciences [1050292] Funding Source: National Science Foundation
Lepista sordida is an edible and medicinal mushroom, but, until now, it had to be collected from the wild. The present study is the first report of the successful cultivation of a wild strain of L. sordida from Thailand. The morphological description and molecular examination of the fungus are included, in order to confirm the identification of the species. Optimization was carried out for mycelium growth and fruiting body production. yeast malt extract (YMA), pH 6.0 - 7.0 and a temperature of 25 - 30 degrees C. A sorghum mushroom spawn was used for upscaling of the mycelium to be used for fruiting body production. Optimal conditions for the fruiting phase were 25 degrees C with 95-97% humidity in a compost rice straw medium with sandy-soil casing layer. Additionally, the secondary metabolites of fruiting body and cultured mycelium were investigated. Nudic acid B, a known toxic polyacetylene, was isolated from submerged cultures of L. sordida, while no polyacetylenic compounds were found in the fruiting bodies.
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