期刊
MOLECULES
卷 22, 期 8, 页码 -出版社
MDPI AG
DOI: 10.3390/molecules22081317
关键词
Woodwardia unigemmata; multidrug resistance; doxorubicin-resistant K562/A02 cells; bovine serum albumin; molecular docking
资金
- Natural Science Foundation of China [81502921]
- Young Scholars Program of Shandong University [2015WLJH50]
Phytochemical investigation on the methanol extract of Woodwardia unigemmata resulted in the isolation of seven flavonoids, including one new flavonol acylglycoside (1). The structures of these compounds were elucidated on the basis of extensive spectroscopic analysis and comparison of literature data. The multidrug resistance (MDR) reversing activity was evaluated for the isolated compounds using doxorubicin-resistant K562/A02 cells model. Compound 6 showed comparable MDR reversing effect to verapamil. Furthermore, the interaction between compounds and bovine serum albumin (BSA) was investigated by spectroscopic methods, including steady-state fluorescence, synchronous fluorescence, circular dichroism (CD) spectroscopies, and molecular docking approach. The experimental results indicated that the seven flavonoids bind to BSA by static quenching mechanisms. The negative Delta H and Delta S values indicated that van derWaals interactions and hydrogen bonds contributed in the binding of compounds 2-6 to BSA. In the case of compounds 1 and 7 systems, the hydrophobic interactions play a major role. The binding of compounds to BSA causes slight changes in the secondary structure of BSA. There are two binding sites of compound 6 on BSA and site I is the main site according to the molecular docking studies and the site marker competitive binding assay.
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