期刊
MOLECULES
卷 22, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/molecules22122253
关键词
gallnut water extract; tannic acid; benzene ring derivatives; biodegradation; Py-GC; MS
资金
- National Key Research & Development Program [2016YFD0600805]
- National Natural Science Foundation of China [31570564]
Gallnut water extract (GWE) enriches 80 similar to 90% of gallnut tannic acid (TA). In order to study the biodegradation of GWE into gallic acid (GA), the LHS-1 strain, a variant of Aspergillus niger, was chosen to determine the optimal degradation parameters for maximum production of GA by the response surface method. Pyrolysis-gas chromatography-mass spectrometry (Py-GC/MS) was first applied to appraise benzene ring derivatives of fermented GWE (FGWE) pyrolysis by comparison with the pyrolytic products of a tannic acid standard sample (TAS) and GWE. The results showed that optimum conditions were at 31 degrees C and pH of 5, with a 50-h incubation period and 0.1 gL-1 of TA as substrate. The maximum yields of GA and tannase were 63 similar to 65 mgmL-1 and 1.17 UmL-1, respectively. Over 20 kinds of compounds were identified as linear hydrocarbons and benzene ring derivatives based on GA and glucose. The key benzene ring derivatives were 3,4,5-trimethoxybenzoic acid methyl ester, 3-methoxy-1,2-benzenediol, and 4-hydroxy-3,5-dimethoxy-benzoic acid hydrazide.
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