期刊
ANALYST
卷 144, 期 1, 页码 206-211出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8an01157e
关键词
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资金
- Eli Lilly and Company's Young Investigator Award
- National Center for Advancing Translational Science (NCATS) [UL1TR001111]
- National Institute of General Medical Sciences (NIGMS) [R35GM129697]
Cellular movement is essential in the formation and maintenance of healthy tissues as well as in disease progression such as tumor metastasis. In this work, we describe a paper-based Transwell assay capable of quantifying cellular invasion through an extracellular matrix. The paper-based Transwell assays generate similar datasets, with equivalent reproducibility, to commercially available Transwell assays. With different culture configurations, we quantify invasion: upon addition of an exogenous factor or in the presence of medium obtained from other cell types, in an indirect or direct co-culture format whose medium composition is dynamically changing, and in a single-zone or parallel (96-zone) format.
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