期刊
MOLECULAR SYSTEMS BIOLOGY
卷 13, 期 4, 页码 -出版社
WILEY
DOI: 10.15252/msb.20167416
关键词
diagnostic bacteria; gut inflammation; tetrathionate; thiosulfate; two-component system
资金
- Welch Foundation [C-1856]
- ONR Young Investigator Award [N00014-14-1-0487]
- NSF [1553317]
- Baylor College of Medicine
- Rice Department of Bioengineering Postdoctoral Fellowship
- [CA1428260]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1553317] Funding Source: National Science Foundation
There is a groundswell of interest in using genetically engineered sensor bacteria to study gut microbiota pathways, and diagnose or treat associated diseases. Here, we computationally identify the first biological thiosulfate sensor and an improved tetrathionate sensor, both two-component systems from marine Shewanella species, and validate them in laboratory Escherichia coli. Then, we port these sensors into a gut-adapted probiotic E. coli strain, and develop a method based upon oral gavage and flow cytometry of colon and fecal samples to demonstrate that colon inflammation (colitis) activates the thiosulfate sensor in mice harboring native gut microbiota. Our thiosulfate sensor may have applications in bacterial diagnostics or therapeutics. Finally, our approach can be replicated for a wide range of bacterial sensors and should thus enable a new class of minimally invasive studies of gut microbiota pathways.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据