期刊
MOLECULAR PLANT PATHOLOGY
卷 19, 期 4, 页码 975-985出版社
WILEY
DOI: 10.1111/mpp.12583
关键词
mushroom-type biofilm; ralfuranones; Ralstonia solanacearum; virulence
资金
- Ministry of Education, Culture, Sports, Science, and Technology, Japan [24580066, 25292029, 26660036, 17H03773, 17K19271]
- Towa Foundation for Food Science Research
- Sumitomo Chemical Co. Ltd.
- Japan Science Society [28-540]
- Grants-in-Aid for Scientific Research [17K19271, 26660036, 17H03773, 24580066, 16H04883] Funding Source: KAKEN
After invasion into intercellular spaces of tomato plants, the soil-borne, plant-pathogenic Ralstonia solanacearum strain OE1-1 forms mushroom-shaped biofilms (mushroom-type biofilms, mBFs) on tomato cells, leading to its virulence. The strain OE1-1 produces aryl-furanone secondary metabolites, ralfuranones (A, B, J, K and L), dependent on the quorum sensing (QS) system, with methyl 3-hydroxymyristate (3-OH MAME) synthesized by PhcB as a QS signal. Ralfuranones are associated with the feedback loop of the QS system. A ralfuranone productivity-deficient mutant (ralA) exhibited significantly reduced growth in intercellular spaces compared with strain OE1-1, losing its virulence. To analyse the function of ralfuranones in mBF formation by OE1-1 cells, we observed cell aggregates of R.solanacearum strains statically incubated in tomato apoplast fluids on filters under a scanning electron microscope. The ralA strain formed significantly fewer microcolonies and mBFs than strain OE1-1. Supplementation of ralfuranones A, B, J and K, but not L, significantly enhanced the development of mBF formation by ralA. Furthermore, a phcB- and ralA-deleted mutant (phcB/ralA) exhibited less formation of mBFs than OE1-1, although a QS-deficient, phcB-deleted mutant formed mBFs similar to OE1-1. Supplementation with 3-OH MAME significantly reduced the formation of mBFs by Delta phcB/ralA. The application of each ralfuranone significantly increased the formation of mBFs by phcB/ralA supplied with 3-OH MAME. Together, our findings indicate that ralfuranones are implicated not only in the development of mBFs by strain OE1-1, but also in the suppression of QS-mediated negative regulation of mBF formation.
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