期刊
MOLECULAR PHARMACEUTICS
卷 14, 期 7, 页码 2450-2458出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.molpharmaceut.7b00352
关键词
nonionic surfactant vesicles; microfluidics; RNA interference; drug delivery
资金
- Jordanian Ministry of Higher Education and Scientific Research in Jordan
- Yarmouk University in Jordan
Small interfering RNAs (siRNA) have a broad potential as therapeutic agents to reversibly silence any target gene of interest. The clinical application of siRNA requires the use of safe and effective delivery systems. In this study, we investigated the use of nonionic surfactant vesicles (NISV) for the delivery of siRNA. Different types of NISV formulations were synthesized by microfluidic mixing and then evaluated for their physiochemical properties and cytotoxicity. The ability:of the NISV to carry and transfect siRNA targeting green fluorescent protein (GFP) into A549 that stably express GFP (copGFP-A549) was evaluated. Flow cytometry and Western blotting were used to study the GFP expression knockdown, and significant knockdown was observed as a result of siRNA delivery to the cells by NISV. This occurred in particular when using Tween 85, which was able to achieve more than 70% GFP knockdown. NISV were thus demonstrated to provide a promising and effective platform for therapeutic delivery of siRNA.
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