期刊
MOLECULAR NUTRITION & FOOD RESEARCH
卷 61, 期 11, 页码 -出版社
WILEY
DOI: 10.1002/mnfr.201700144
关键词
DSS mouse colitis; Microbiota; MicroRNA; Probiotic; Pyrosequencing
资金
- Junta de Andalucia [CTS 164]
- Spanish Ministry of Economy and Competitiveness [SAF2011-29648, AGL2015-67995-C3-3-R]
- European Union
- Instituto de Salud Carlos III
Scope: To compare the intestinal anti-inflammatory effects of two probiotics Lactobacillus fermentum and Lactobacillus salivarius in mouse colitis, focusing on their impact on selected miRNAs and microbiota composition. Methods and results: Male C57BL/6J mice were randomly assigned to four groups (n = 10): non-colitic, DSS colitic and two colitic groups treated with probiotics (5 x 10(8) CFU/mouse/day). Both probiotics ameliorated macroscopic colonic damage. They improved the colonic expression of markers involved in the immune response, and the expression of miR-155 and miR-223. L. fermentum also restored miR-150 and miR-143 expression, also linked to the preservation of the intestinal barrier function. Besides, these beneficial effects were associated with the amelioration of the microbiota dysbiosis and a recovery of the SCFAs- and lactic acid-producing bacterial populations, although only L. fermentum improved Chao richness, Pielou evenness and Shannon diversity. Moreover, L. fermentum also restored the Treg cell population in MLNs and the Th1/Th2 cytokine balance. Conclusion: Both probiotics exerted intestinal anti-inflammatory effects in DSS-mouse colitis, maybe due to their ability to restore the intestinal microbiota homeostasis and modulate the immune response. L. fermentum showed a greater beneficial effect compared to L. salivarius, which makes it more interesting for future studies.
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