期刊
MOLECULAR NUTRITION & FOOD RESEARCH
卷 61, 期 7, 页码 -出版社
WILEY
DOI: 10.1002/mnfr.201600807
关键词
Grain Composition; Metabolomics; NMR Spectroscopy; Transgenic; Wheat
资金
- European Commission in the Community's 6th Framework Programme
- Biotechnology and Biological Sciences Research Council of the United Kingdom
- [BB/G004781/1]
- Biotechnology and Biological Sciences Research Council [BBS/E/C/00004976, BBS/E/C/00004158, BB/L017768/1, BB/E015794/1, BB/G004781/1, BBS/E/W/0012843D, BBS/E/C/00004975, 1096616] Funding Source: researchfish
- BBSRC [BB/G004781/1, BBS/E/C/00004975, BBS/E/W/0012843D, BBS/E/C/00004976, BB/L017768/1, BB/E015794/1] Funding Source: UKRI
ScopeThe application of high-throughput 1H nuclear magnetic resonance (1H-NMR) of unpurified extracts to determine genetic diversity and the contents of polar components in grain of wheat. Methods and resultsMilled whole wheat grain was extracted with 80:20 D2O:CD3OD containing 0.05% d(4)-trimethylsilylpropionate. 1H-NMR spectra were acquired under automation at 300 degrees K using an Avance Spectrometer operating at 600.0528 MHz. Regions for individual metabolites were identified by comparison to a library of known standards run under identical conditions. The individual 1H-NMR peaks or levels of known metabolites were then compared by Principal Component Analysis using SIMCA-P software. ConclusionsHigh-throughput 1H-NMR is an excellent tool to compare the extent of genetic diversity within and between wheat species, and to quantify specific components (including glycine betaine, choline, and asparagine) in individual genotypes. It can also be used to monitor changes in composition related to environmental factors and to support comparisons of the substantial equivalence of transgenic lines.
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