期刊
MOLECULAR MICROBIOLOGY
卷 104, 期 2, 页码 319-333出版社
WILEY
DOI: 10.1111/mmi.13629
关键词
-
资金
- VIDI fellowship from the Netherlands Organisation for Scientific Research [864.09.010]
- ERC starting grant [337399-PneumoCell]
- Chinese Scholarship Council
- POPH/FSE and FCT (Fundacao para a Ciencia e Tecnologia) from Portugal [SFRH/BD/78061/2011]
- Dutch Ministry of Education, Culture and Science [024.001.035]
- VIDI grant from the Netherlands Organisation for Scientific Research (NWO-CW) [723.014.008]
- EMBO Young Investigator Program, a VIDI fellowship from the Netherlands Organisation for Scientific Research [864.12.001]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/78061/2011] Funding Source: FCT
Peptidoglycan (PG), the major component of the bacterial cell wall, is one large macromolecule. To allow for the different curvatures of PG at cell poles and division sites, there must be local differences in PG architecture and eventually also chemistry. Here we report such local differences in the Gram-positive rod-shaped model organism Bacillus subtilis. Single-cell analysis after antibiotic treatment and labeling of the cell wall with a fluorescent analogue of vancomycin or the fluorescent D-amino acid analogue (FDAA) HCC-amino-D-alanine revealed that PG at the septum contains muropeptides with unprocessed stem peptides (pentapeptides). Whereas these pentapeptides are normally shortened after incorporation into PG, this activity is reduced at division sites indicating either a lower local degree of PG crosslinking or a difference in PG composition, which could be a topological marker for other proteins. The pentapeptides remain partially unprocessed after division when they form the new pole of a cell. The accumulation of unprocessed PG at the division site is not caused by the activity of the cell division specific penicillin-binding protein 2B. To our knowledge, this is the first indication of local differences in the chemical composition of PG in Gram-positive bacteria.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据