期刊
MOLECULAR MEDICINE REPORTS
卷 17, 期 2, 页码 3281-3287出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2017.8218
关键词
grifolic acid; macrophage; mitochondria; cell death; free fatty acid receptor 4
资金
- Shaanxi Province [2015KTCQ03-03]
- Xi'an Medical University [2015RCYJ02]
Grifolic acid is a phenolic compound that was first extracted from the mushroom Albatrellus confluens; it acts as an agonist of the free fatty acid receptor (FFAR4). FFAR4 is expressed in macrophages and mediates the anti-inflammatory effects of n-3 unsaturated free fatty acids. In the present study, the effects of grifolic acid on macrophages were observed in mouse RAW264.7 cells. It was demonstrated that grifolic acid (2.5-20 mu mol/l) treatment reduced RAW264.7 cell viability in a dose- and time-dependent manner. The number of apoptotic cells significantly increased following grifolic acid treatment compared with the untreated control cells. Grifolic acid treatment resulted in a significant decrease in cellular adenosine 5'-triphosphate (ATP) content in RAW264.7 cells. Mitochondrial membrane potential (MMP), as measured by JC-1 staining, was significantly diminished by grifolic acid treatment in a dose- and time-dependent manner. Treatment with cyclosporine A, a protector of MMP, attenuated grifolic acid-induced reduction of MMP and viability in RAW264.7 cells. FFAR4 knockdown did not significantly influence grifolic acid-induced reduction of cell viability, ATP levels or MMP. In conclusion, grifolic acid may induce macrophage cell death by reducing MMP and by inhibiting ATP production probably in an FFAR4-independent manner.
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