期刊
MOLECULAR MEDICINE REPORTS
卷 16, 期 3, 页码 2907-2914出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2017.6943
关键词
caspase-14; filaggrin; ginsenoside Rc; glutathione; reactive oxygen species; skin barrier function; superoxide dismutase; UVB radiation
资金
- Ministry of Trade, Industry and Energy, Korea Institute for Advancement of Technology, through the Encouragement Program for the Industries of Economic Cooperation Region [R0005382]
- Korea Evaluation Institute of Industrial Technology (KEIT) [R0005382] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ginsenoside Rc (Rc) is a major ginsenoside isolated from Panax ginseng, and has exhibited pharmacological effects on skin cells. The present study aimed to investigate the putative skin-protective properties of Rc, including its anti-photoaging and barrier function-protective effects, in human HaCaT keratinocytes exposed to UVB radiation. The protective properties of Rc were evaluated through the assessment of keratinocyte viability, reactive oxygen species (ROS) production, total glutathione (GSH) and superoxide dismutase (SOD) activity, caspase-14, matrix metalloproteinase (MMP)-2 and -9 activity, and MMP-2, MMP-9 and filament aggregating protein (filaggrin) expression following UVB irradiation. Treatment with Rc was revealed to prevent the UVB-induced increase in ROS production and pro-MMP-2 and -9 levels in HaCaT keratinocytes. In addition, treatment with Rc resulted in enriched GSH contents and enhanced SOD activity following exposure to UVB radiation. Furthermore, Rc treatment enhanced caspase-14 activity and counteracted the UVB-induced downregulation in filaggrin expression. However, no significant difference was identified between Rc-treated and normal groups in terms of keratinocyte viability, regardless of exposure to radiation. The present findings suggested that Rc may exert anti-photoaging and barrier function-protective effects in keratinocytes, and thus protect the skin against photooxidative stress induced by exposure to UV radiation.
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