期刊
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
卷 166, 期 2, 页码 B49-B55出版社
ELECTROCHEMICAL SOC INC
DOI: 10.1149/2.0541902jes
关键词
-
资金
- National Natural Science Foundation of China [21775122, 21775123, 21705115, 51473136, 21575116]
- Natural Science Foundation of Chongqing City [cstc2018jcyjAX0693]
- Natural Science Foundation of Jiangsu Province of China [BK20170378]
- Natural Science Research Foundation of Jiangsu Higher Education Institutions [17KJB150036]
A sandwich electrochemiluminescence (ECL) biosensing system for soybean agglutinin (SBA) detection was developed with AuPt@C-60 nanoflowers (AuPt@C-60 NFs) as a novel co-reactant accelerator to enhance the performance of N-(aminobutyl)-N-(ethylisoluminol) (ABEI). In this strategy, nanoflower-liked AuPt@C-60 complex served as a matrix for loading a large sum of ABEI and galactosamine (Gal) by Au-N and Pt-N bonds to achieve the nanocomposite Gal-ABEI-AuPt@C-60 NFs. Dissolved oxygen was used as a co-reaction reagent, while AuPt@C-60 complex acted as a co-reactant accelerator for dissolved oxygen to enhanced the ECL emission of ABEI. Gal, as an identifying element, was highly loaded by electrodeposited gold nanoparticles (Dp Au) on the electrode to recognize SBA, and further combine with Gal-ABEI-AuPt@C-60 NFs to form a sandwich model. As a result, the proposed SBA biosensor exhibited a wide linear range from 1.0 ng . mL(-1) to 5.0 mu g . mL(-1) and a low detection limit of 0.33 ng . mL(-1). In addition, the biosensor displayed an excellent selectivity and an acceptable stability and reproducibility. The combination of AuPt@C-60 NFs and ABEI provided a new ECL sensing platform without exogenous co-reactant. (C) 2019 The Electrochemical Society.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据