期刊
BREAST CANCER RESEARCH
卷 21, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s13058-018-1071-2
关键词
Tumor-initiating cells; Hydrodynamic shear stress; ROS; NO; EMT; MET; ERK-GSK3
类别
资金
- National Research Foundation (NRF) - Korean government [2013M3A9D3045880, 2015R1A5A1009701, 2017M3A9C602956, NRF-2017R1A2B3004828]
BackgroundEpithelial-mesenchymal transition (EMT) occurs in the tumor microenvironment and presents an important mechanism of tumor cell intravasation, stemness acquisition, and metastasis. During metastasis, tumor cells enter the circulation to gain access to distant tissues, but how this fluid microenvironment influences cancer cell biology is poorly understood.Methods and resultsHere, we present both in vivo and in vitro evidence that EMT-like transition also occurs in circulating tumor cells (CTCs) as a result of hydrodynamic shear stress (+SS), which promotes conversion of CD24(middle)/CD44(high)/CD133(middle)/CXCR4(low)/ALDH1(low) primary patient epithelial tumor cells into specific high sphere-forming CD24(low)/CD44(low)/CD133(high)/CXCR4(high)/ALDH1(high) cancer stem-like cells (CSLCs) or tumor-initiating cells (TICs) with elevated tumor progression and metastasis capacity in vitro and in vivo. We demonstrate that conversion of CSLCs/TICs from epithelial tumor cells via+SS is dependent on reactive oxygen species (ROS)/nitric oxide (NO) generation, and suppression of extracellular signal-related kinase (ERK)/glycogen synthase kinase (GSK)3, a mechanism similar to that operating in embryonic stem cells to prevent their differentiation while promoting self-renewal.ConclusionFluid shear stress experienced during systemic circulation of human breast tumor cells can lead to specific acquisition of mesenchymal stem cell (MSC)-like potential that promotes EMT, mesenchymal-epithelial transition, and metastasis to distant organs. Our data revealed that biomechanical forces appeared to be important microenvironmental factors that not only drive hematopoietic development but also lead to acquisition of CSLCs/TIC potential in cancer metastasis. Our data highlight that +SS is a critical factor that promotes the conversion of CTCs into distinct TICs in blood circulation by endowing plasticity to these cells and by maintaining their self-renewal signaling pathways.
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