期刊
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
卷 60, 期 1, 页码 192-201出版社
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
DOI: 10.1167/iovs.18-25721
关键词
chemokine; complement; interferon; LCMV; retina; RPE
资金
- Lundbeckfonden
- Aase og Ejnar Danielsens Fond
- Danish Eye Research Foundation
- Fight for Sight Denmark
PURPOSE. To examine how circulating immune mediators in vivo may affect gene and protein expression at the RPE/choroid interface. METHODS. Young mice were systemically infected with lymphocytic choriomeningitis virus (LCMV) or continuously infused with IFN-gamma. RPE/choroid was isolated and analyzed with whole-transcriptome gene expression microarrays. Selected gene expression findings were validated at the protein level. RESULTS. Both the systemic immune activation from virus infection and the sterile systemically increased level of IFN-gamma resulted in increased expression of chemokine ligands, chemokine receptors, and early complement components in isolates of RPE/choroid. These findings were largely absent from LCMV-infected mice deficient in either the interferon alpha/beta receptor or IFN-gamma. CONCLUSIONS. Together, these findings demonstrate that acute systemic immune activation results in a local response at the RPE/choroid interface that may include chemokinedependent recruitment of inflammatory cells and engagement of the complement system. This may represent a link between the systemic low-grade inflammation and the retinal pathology observed in several multifactorial entities such as aging, AMD, and diabetes.
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