期刊
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
卷 60, 期 1, 页码 183-191出版社
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
DOI: 10.1167/iovs.18-25861
关键词
calcium imaging; retinal ganglion cells; Thy1-GCaMP3; GCaMP3; optic nerve transection
资金
- Atlantic Canada Opportunities Agency (ACOA) Atlantic Innovation Fund (AIF) [197809]
- Nova Scotia Health Research Foundation
- Canadian Institutes of Health Research (CIHR)
PURPOSE. GCaMP3 is a genetically encoded calcium indicator for monitoring intracellular calcium dynamics. We characterized the expression pattern and functional properties of GCaMP3 in the Thy1-GCaMP3 transgenic mouse retina. METHODS. To determine the specificity of GCaMP3 expression, Thy1-GCaMP3 (B6; CBATg( Thy1-GCaMP3) 6Gfng/ J) retinas were processed for immunohistochemistry with anti-green fluorescent protein (anti-GFP, to enhance GCaMP3 fluorescence), anti-RBPMS (retinal ganglion cell [RGC]-specific marker), and antibodies against amacrine cell markers (ChAT, GABA, GAD67, syntaxin). Calcium imaging was used to characterize functional responses of GCaMP3-expressing (GCaMPthorn) cells by recording calcium transients evoked by superfusion of kainic acid (KA; 10, 50, or 100 lM). In a subset of animals, optic nerve transection (ONT) was performed 3, 5, or 7 days prior to calcium imaging. RESULTS. GFP immunoreactivity colocalized with RBPMS but not amacrine cell markers in both ONT and non-ONT (control) groups. Calcium transients evoked by KA were reduced after ONT (50 lM KA; DF/ F0 [SD]; control: 1.00 [0.67], day 3: 0.50 [0.35], day 5: 0.31 [0.28], day 7: 0.35 [0.36]; P < 0.05 versus control). There was also a decrease in the number of GCaMP3thorn cells after ONT (cells/ mm 2 [SD]; control: 2198 [453], day 3: 2224 [643], day 5: 1383 [375], day 7: 913 [178]; P < 0.05). Furthermore, the proportion of GCaMP3thorn cells that responded to KA decreased after ONT (50 lM KA, 97%, 54%, 47%, and 58%; control, 3, 5, and 7 days, respectively). CONCLUSIONS. Following ONT, functional RGC responses are lost prior to the loss of RGC somata, suggesting that anatomical markers of RGCs may underestimate the extent of RGC dysfunction.
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