期刊
MOLECULAR CELL
卷 65, 期 1, 页码 25-38出版社
CELL PRESS
DOI: 10.1016/j.molcel.2016.11.029
关键词
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资金
- European Research Council [339270]
- Wellcome Trust [107928/Z/15/Z, 090532/Z/09/Z]
- Fundacao para a Ciencia e Tecnologia, Portugal [PTDC/BEX-BCM/5899/2014]
- Fundação para a Ciência e a Tecnologia [PTDC/BEX-BCM/5899/2014] Funding Source: FCT
- European Research Council (ERC) [339270] Funding Source: European Research Council (ERC)
Numerous long intervening noncoding RNAs (lincRNAs) are generated from the mammalian genome by RNA polymerase II (Pol II) transcription. Although multiple functions have been ascribed to lincRNAs, their synthesis and turnover remain poorly characterized. Here, we define systematic differences in transcription and RNA processing between protein-coding and lincRNA genes in human HeLa cells. This is based on a range of nascent transcriptomic approaches applied to different nuclear fractions, including mammalian native elongating transcript sequencing (mNET-seq). Notably, mNET-seq patterns specific for different Pol II CTD phosphorylation states reveal weak co-transcriptional splicing and poly(A) signal-independent Pol II termination of lincRNAs as compared to pre-mRNAs. In addition, lincRNAs are mostly restricted to chromatin, since they are rapidly degraded by the RNA exosome. We also show that a lincRNA-specific co-transcriptional RNA cleavage mechanism acts to induce premature termination. In effect, functional lincRNAs must escape from this targeted nuclear surveillance process.
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