期刊
MOLECULAR CELL
卷 66, 期 1, 页码 77-+出版社
CELL PRESS
DOI: 10.1016/j.molcel.2017.02.023
关键词
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资金
- Boston-area Research Training Program in Biomedical Informatics [T15LM007092]
- Deutsche Forschungsgemeinschaft Fellowship through the Graduate School of Quantitative Biosciences Munich (QBM) [GSC 1006]
- Deutsche Forschungsgemeinschaft [SFB860, SPP1935]
- European Research Council Advanced Grant TRANSREGULON [693023]
- Volkswagen Foundation
- William F. Milton Fund
- Harvard Medical School
- NIH [GM032967]
Spt5 is an essential and conserved factor that functions in transcription and co-transcriptional processes. However, many aspects of the requirement for Spt5 in transcription are poorly understood. We have analyzed the consequences of Spt5 depletion in Schizosaccharomyces pombe using four genome-wide approaches. Our results demonstrate that Spt5 is crucial for a normal rate of RNA synthesis and distribution of RNAPII over transcription units. In the absence of Spt5, RNAPII localization changes dramatically, with reduced levels and a relative accumulation over the first similar to 500 bp, suggesting that Spt5 is required for transcription past a barrier. Spt5 depletion also results in widespread antisense transcription initiating within this barrier region. Deletions of this region alter the distribution of RNAPII on the sense strand, suggesting that the barrier observed after Spt5 depletion is normally a site at which Spt5 stimulates elongation. Our results reveal a global requirement for Spt5 in transcription elongation.
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