期刊
MOLECULAR CELL
卷 68, 期 5, 页码 1006-+出版社
CELL PRESS
DOI: 10.1016/j.molcel.2017.11.017
关键词
-
资金
- National Human Genome Research Institute (NHGRI) [R00HG007982]
- National Heart Lung and Blood Institute (NHLBI) [DP2HL142044]
- Penn Epigenetics Institute Pilot Grant
- National Institute of Mental Health (NIMH) [R56MH111719]
- T32 Training Program in Cell and Molecular Biology [T32-GM007229]
- T32 Training Program in Neurodevelopmental Disabilities [T32-NS007413]
- NRAS Individual Predoctoral Fellowship [F30-NS100433]
Massively parallel single-cell RNA sequencing can precisely resolve cellular diversity in a high-throughput manner at low cost, but unbiased isolation of intact single cells from complex tissues such as adult mammalian brains is challenging. Here, we integrate sucrose-gradient-assisted purification of nuclei with droplet microfluidics to develop a highly scalable single-nucleus RNA-seq approach (sNucDrop-seq), which is free of enzymatic dissociation and nucleus sorting. By profiling similar to 18,000 nuclei isolated from cortical tissues of adult mice, we demonstrate that sNucDrop-seq not only accurately reveals neuronal and non-neuronal subtype composition with high sensitivity but also enables in-depth analysis of transient transcriptional states driven by neuronal activity, at single-cell resolution, in vivo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据