期刊
MOLECULAR BREEDING
卷 37, 期 6, 页码 -出版社
SPRINGER
DOI: 10.1007/s11032-017-0675-z
关键词
Bioenergy crop; Cross-amplification; Erianthus arundinaceus; Microsatellites; Saccharum complex; SSR
资金
- National Agriculture and Food Research Organization [220a0]
- Japan International Research Center for Agricultural Sciences [001750]
Erianthus arundinaceus, a member of the Saccharum complex, is of interest as a potential resource for sugarcane improvement and as a bioenergy crop. Genetic analyses of germplasm collections of E. arundinaceus are being used increasingly. To expand the genomic resources in E. arundinaceus, we aimed at developing simple sequence repeat markers. Using pyrosequencing on the 454 GS FLX system, we sequenced genomic DNA from JW630 collected in Japan. A total of 1682 candidate loci were used to design the primers, and 1234 primer pairs amplified fragments of the expected size in the primer screening with three wild E. arundinaceus accessions (JW630, JW4, and IJ76-349). The efficiency of genotyping was validated with a subset of 174 primer pairs and 8 E. arundinaceus accessions. Of these primer pairs, 171 amplified fragments in all accessions tested and 162 detected polymorphic loci. The average values of genetic parameters were estimated as 0.30 (range, 0.09-0.49) for polymorphic information content, 1.65 (0.00-5.87) for marker index, and 2.78 (0.00-8.75) for resolving power. Using these parameters, we selected 61 primer pairs with large discriminatory power for the analyzed loci. Of the 174 primer pairs, 45 (25.9%) were also applicable to Saccharum and 33 (19.0%) to Miscanthus species. These markers would provide a valuable tool for estimating genetic diversity and constructing linkage maps in E. arundinaceus, which would be useful for genetic study and breeding.
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