期刊
MOLECULAR BIOTECHNOLOGY
卷 59, 期 7, 页码 241-250出版社
HUMANA PRESS INC
DOI: 10.1007/s12033-017-0014-y
关键词
Gossypium hirsutum; Tobacco rattle virus vector; Virus-induced gene silencing; Enoyl-CoA reductase; Cotton wilt
资金
- Higher Education Commission (HEC) Govt. of Pakistan under the International Research Support Initiative Program (IRSIP)
- Pak-US cotton productivity enhancement program of the International Center for Agricultural Research in the Dry Areas (ICARDA) - United States Department of Agriculture (USDA), Agricultural Research Service (ARS) [58-6402-0-178F]
A Tobacco rattle virus (TRV)-based virus-induced gene silencing assay was employed as a reverse genetic approach to study gene function in cotton (Gossypium hirsutum). This approach was used to investigate the function of the Enoyl-CoA reductase (GhECR) gene in pathogen defense. Amino acid sequence alignment of Arabidopsis ECR with homologous sequence from G. hirsutum, G. arboreum, G. herbaceum and G. barbadense showed that ECRs are highly conserved among these species. TRV-based silencing of GhECR gene in G. hirsutum induced a cell death/necrotic lesion-like phenotype. Reverse transcription polymerase chain reaction (RT-PCR) and real-time quantitative PCR showed reduced GhECR mRNA levels in TRV inoculated plants. Three isolates of Verticillium dahliae (V. dahliae) and Fusarium oxysporum f. sp. vasinfectum (FOV) were used to infect GhECR-silenced plants. Out of 6 races of 2 pathogens, down regulation of GhECR gene resulted in reduced resistance. This is the first report showing that cotton GhECR gene is involved in resistance to different strains of V. dahliae and FOV.
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