期刊
MOLECULAR BIOLOGY OF THE CELL
卷 28, 期 8, 页码 1021-1033出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E16-06-0461
关键词
-
类别
资金
- NIGMS NIH HHS [R01 GM062290, P41 GM103313, R01 GM095977] Funding Source: Medline
- Div Of Civil, Mechanical, & Manufact Inn
- Directorate For Engineering [1361987] Funding Source: National Science Foundation
Dendritic filopodia are actin-filled dynamic subcellular structures that sprout on neuronal dendrites during neurogenesis. The exploratory motion of the filopodia is crucial for synaptogenesis, but the underlying mechanisms are poorly understood. To study filopodial motility, we collected and analyzed image data on filopodia in cultured rat hippocampal neurons. We hypothesized that mechanical feedback among the actin retrograde flow, myosin activity, and substrate adhesion gives rise to various filopodial behaviors. We formulated a minimal one-dimensional partial differential equation model that reproduced the range of observed motility. To validate our model, we systematically manipulated experimental correlates of parameters in the model: substrate adhesion strength, actin polymerization rate, myosin contractility, and the integrity of the putative microtubule-based barrier at the filopodium base. The model predicts the response of the system to each of these experimental perturbations, supporting the hypothesis that our actomyosin-driven mechanism controls dendritic filopodia dynamics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据