期刊
MOLECULAR BIOLOGY AND EVOLUTION
卷 34, 期 5, 页码 1167-1182出版社
OXFORD UNIV PRESS
DOI: 10.1093/molbev/msx066
关键词
bacterial population genetics; recombination detection; population structure; hidden Markov models; Streptococcus pneumoniae; antibiotic resistance
资金
- Academy of Finland [286607, 294015]
- Junior Research Fellowship from Imperial College London
- Medical Research Council [MR/K010174/1B] Funding Source: researchfish
- Academy of Finland (AKA) [294015, 286607, 286607, 294015] Funding Source: Academy of Finland (AKA)
Prokaryotic evolution is affected by horizontal transfer of genetic material through recombination. Inference of an evolutionary tree of bacteria thus relies on accurate identification of the population genetic structure and recombination-derived mosaicism. Rapidly growing databases represent a challenge for computational methods to detect recombinations in bacterial genomes. We introduce a novel algorithm called fastGEAR which identifies lineages in diverse microbial alignments, and recombinations between them and from external origins. The algorithm detects both recent recombinations (affecting a few isolates) and ancestral recombinations between detected lineages (affecting entire lineages), thus providing insight into recombinations affecting deep branches of the phylogenetic tree. In simulations, fastGEAR had comparable power to detect recent recombinations and outstanding power to detect the ancestral ones, compared with state-of-the-art methods, often with a fraction of computational cost. We demonstrate the utility of the method by analyzing a collection of 616 whole-genomes of a recombinogenic pathogen Streptococcus pneumoniae, for which the method provided a high-resolution view of recombination across the genome. We examined in detail the penicillin-binding genes across the Streptococcus genus, demonstrating previously undetected genetic exchanges between different species at these three loci. Hence, fastGEAR can be readily applied to investigate mosaicism in bacterial genes across multiple species. Finally, fastGEAR correctly identified many known recombination hotspots and pointed to potential new ones. Matlab code and Linux/Windows executables are available at https://users.ics.aalto.fi/similar to pemartti/fastGEAR/ (last accessed February 6, 2017).
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