4.2 Article

Studies on Bacterial Diversity and Vibrio harveyi Distribution Associated with Diseased fugu (Takifugu rubripes) in Northeastern China

期刊

PAKISTAN JOURNAL OF ZOOLOGY
卷 51, 期 1, 页码 67-77

出版社

ZOOLOGICAL SOC PAKISTAN
DOI: 10.17582/journal.pjz/2019.51.1.67.77

关键词

Takifugu rubripes; Bacterial diversity; Vibrio harveyi

类别

资金

  1. National Public Science and Technology Research Funds Projects of Ocean [201405003]
  2. National key R&D Program of China [2017YFD0701700]
  3. Planned Science and Technology Project of Liaoning [2017203002]

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This study investigated the bacterial diversity and Vibrio harveyi distribution associated with diseased fugu (Takifugu rubripes) in northeastern China from January to December in 2014. The main clinical signs included fin ulceration, skin darkness, hepatohemia and intestinal hydrops. Totally, 104 diseased live fish were collected and 70 strains isolated from naturally diseased T. rubripes. Most isolates were obtained in May, September and December. The isolates were identified through 16S rRNA gene sequence analysis and Vibrio spp.-specific PCR amplification, followed by pathogenicity determination. Results showed that the isolates belonged to 10 genera, including Vibrio (72%), Staphylococcus (9%), Pseudomonas (4%), Bacillus (4%), Vagococcus (3%), Shewanella (3%), Planococcus migula (4%), Exiguobacterium (1%), Enterobacter (1%) and Kocuria roseus (1%). Vibrio spp. and Vibrio harveyi were the predominant genus and species, respectively. In addition, challenge tests demonstrated that 13 out of 70 isolates were strongly pathogenic and identified as V. harveyi. This study illustrated that V. harveyi could be considered as main pathogen. These investigation results would provide useful information for disease prevention in T. rubripes culture.

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