期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 439, 期 C, 页码 116-125出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2016.10.029
关键词
Eicosapentaenoic acid; Lipopolysaccharide; Myotubes; Insulin; Mammalian target of rapamycin
资金
- National Program on Key Basic research Project [2012CB127305]
- Special Fund for Agroscientific Research in the Public Interest [201003060]
- Hubei Provincial Creative Team Project of Agricultural Science and Technology [2007-620]
- Hubei Provincial Natural Science Foundation [2013CFA010]
- Fundamental Research Funds for the Central Universities [2013PY047, 2013QC004]
Dietary n-3 polyunsaturated fatty acids (n-3 PUFAs) increase insulin signaling in skeletal muscle. In the current study, we investigated the effect of eicosapentaenoic acid (EPA) on insulin-induced mammalian target of rapamycin (mTOR) phosphorylation in myotubes. We showed that EPA did not affect basal and insulin-induced mTOR phosphorylation in myotubes. However, EPA abolished lipopolysaccharide (LPS)-induced deficiency in insulin signaling (P < 0.05). Pre-incubation of nuclear factor kappa B (NF-kappa B) and c-Jun N-terminal kinases (JNK) inhibitors prevented the decreased insulin-induced mTOR phosphorylation elicited by LPS (P < 0.05). In addition, in protein tyrosine phosphatase-1B (PTP1B) knockdown myotubes, LPS failed to decrease insulin-induced mammalian target of rapamycin (mTOR) phosphorylation in myotubes (P > 0.05). In myotubes, LPS stimulated PTP1B expression via NF-kappa B and activation protein-1 (AP1). Pre-incubation of 50 mu M EPA prevented the LPS-induced activation of AP1 and NF-kappa B as well as PTP1B expression (P < 0.05). Interestingly, incubation of peroxisome proliferator-activated receptor gamma (PPAR gamma) antagonist (GW9662) prior to EPA treatment, the effect of EPA on insulin-induced mTOR phosphorylation was blocked. Accordingly, EPA did not inhibit the LPS-induced activation of AP1 or NF kappa B as well as PTP1B expression when incubation of GW9662 prior to EPA treatment. The in vivo study showed that EPA prevented LPS-induced PTPTIB expression and a decrease in insulin-induced mTOR phosphorylation in muscle of mice. In summary, EPA abolished LPS inhibition of insulin-induced mTOR phosphorylation in myotubes, and one of the key mechanisms was to inhibit AP1 and NF-kappa B activation and PTP1B transcription. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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