期刊
MOLECULAR & CELLULAR TOXICOLOGY
卷 13, 期 4, 页码 405-417出版社
KOREAN SOCIETY TOXICOGENOMICS & TOXICOPROTEOMICS-KSTT
DOI: 10.1007/s13273-017-0045-2
关键词
Fucoidan; Anti-inflammation; ROS; Macrophages; Zebrafish
资金
- Ministry of Oceans and Fisheries [20150242]
- National Marine Biodiversity Institute Research Program [2017M01400]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Korea government [2016R1A5A2007009]
Fucoidan, a sulfated polysaccharide, is an active component found in various species of seaweed. Although this compound has a strong anti-inflammatory activity, the underlying mechanisms exerted by fucoidan have not been fully elucidated. In the present study, the anti-inflammatory effects of fucoidan on lipopolysaccharide (LPS)-stimulated macrophages and zebrafish larvae were examined. The present data indicated that fucoidan significantly suppressed the secretion of pro-inflammatory mediators including nitric oxide (NO) and prostaglandin E-2 (PGE(2)), and cytokines, such as tumor necrosis factor-alpha and interleukin-1 beta in RAW 264.7 macrophages without any significant cytotoxicity, the protective effects of which were accompanied by a marked reduction in their regulatory gene expression at the transcription levels. Fucoidan also inhibited translocation of the nuclear factor-kappa B from the cytoplasm to the nucleus and attenuated LPS-induced production of intracellular reactive oxygen species (ROS) in RAW 264.7 macrophages. Moreover, fucoidan reduced NO and PGE(2) production and ROS accumulation in LPS-stimulated zebrafish larvae, which was associated with a diminished recruitment of neutrophils and macrophages. Based on the results of this study, we suggest that fucoidan has excellent potential as a therapeutic agent for inflammatory disorders.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据