期刊
MOLECULAR & CELLULAR PROTEOMICS
卷 17, 期 2, 页码 357-372出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/mcp.M116.065755
关键词
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资金
- Region Hauts de France (CM/YB) [2015.2097/12]
- PROTEO [FRQNT-RS-188158]
- University Lille 1 (BQR)
- University Lille 1
- Region Hauts de France [NEU/2015058]
- Canadian Institutes for Health Research [MOP-136962]
- Canada Research Chairs in Functional Proteomics and Discovery of New Protein
- PRISM
- Ministere de l'Enseignement Superieur et de la Recherche via Institut Universitaire de France
- SIRIC ONCOLille
- [INCa-DGOS-Inserm 6041]
Tissue spatially-resolved proteomics was performed on 3 brain regions, leading to the characterization of 123 reference proteins. Moreover, 8 alternative proteins from alternative open reading frames (AltORF) were identified. Some proteins display specific post-translational modification profiles or truncation linked to the brain regions and their functions. Systems biology analysis performed on the proteome identified in each region allowed to associate sub-networks with the functional physiology of each brain region. Back correlation of the proteins identified by spatially-resolved proteomics at a given tissue localization with the MALDI MS imaging data, was then performed. As an example, mapping of the distribution of the matrix metallopeptidase 3-cleaved C-terminal fragment of -synuclein (aa 95-140) identified its specific distribution along the hippocampal dentate gyrus. Taken together, we established the molecular physiome of 3 rat brain regions through reference and hidden proteome characterization.
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